Number of tRNA binding sites on 80 S ribosomes and their subunits
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Number of tRNA binding sites on 80 S ribosomes and their subunits.
The ability of rabbit liver ribosomes and their subunits to form complexes with different forms of tRNAPhe (aminoacyl-, peptidyl- and deacylated) was studied using the nitrocellulose membrane filtration technique. The 80 S ribosomes were shown to have two binding sites for aminoacyl- or peptidyl-tRNA and three binding sites for deacylated tRNA. The number of tRNA binding sites on 80 S ribosomes...
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Programmed 30 S subunits expose only one binding site, to which the different classes of tRNA (deacylated tRNAPhe, Phe-tRNAPhe, and N-acetylphenylalanyl (AcPhe)-tRNAPhe) bind with about the same affinity. Elongation factor Tu within the ternary complex does not contribute to the binding of Phe-tRNA. Binding of acylated or deacylated tRNA to 30 S depends on the cognate codon; nonprogrammed 30 S ...
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The interaction between tRNA and rabbit liver 80S ribosomes and 40S subunits was studied using a nitrocellulose membrane filtration technique. Binding of the different tRNA forms (aminoacyl-, peptidyl- or deacylated) to poly(U)-programmed 40S subunits and 80S ribosomes was found to be a cooperative process. The association constants of AcPhe-TRNA(Phe) for the A and P sites of 80S ribosomes and ...
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A class of 80 S ribosomes has been isolated from purified yeast mitochondria. These ribosomes are distinguished from cytoplasmic ribosomes of the postmitochondrial supernatant fraction by having a greater stability against the dissociating effect of 0.4 M KCI. These results confirm observations of Schmitt ((1969) Fed. Eur. Biochem. Sot. Left. 4, 234-238). Ribosome dissociability has been examin...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1988
ISSN: 0014-5793
DOI: 10.1016/0014-5793(88)80705-1